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Xiaochen LIU, Hu LI, Yao CAI, Fan SONG, John-James WILSON, Wanzhi CAI
《农业科学与工程前沿（英文）》 2017年 第4卷 第3期 页码 358-365 doi: 10.15302/J-FASE-2017158
关键词： gene rearrangement mitochondrial genome Psocoptera Stenopsocidae TDRL model
Improving prodeoxyviolacein production via multiplex SCRaMbLE iterative cycles
Juan Wang, Bin Jia, Zexiong Xie, Yunxiang Li, Yingjin Yuan
《化学科学与工程前沿（英文）》 2018年 第12卷 第4期 页码 806-814 doi: 10.1007/s11705-018-1739-2
The synthetic chromosome rearrangement and modification by loxP-mediated evolution (SCRaMbLE) system has been used to improve prodeoxyviolacein (PDV) production in haploid yeast containing chromosome synV. To rapidly and continuously generate genome diversification with the desired phenotype, the multiplex SCRaMbLE iterative cycle strategy has been developed for the screening of high PDV production strains. Whole-genome sequencing analysis reveals large duplications, deletions, and even the whole genome duplications. The deletion of YER151C is proved to be responsible for the increase. This study demonstrates that artificial DNA rearrangement can be used to accelerate microbial evolution and the production of biobased chemicals.
关键词： synthetic biology genome rearrangement prodeoxyviolacein SCRaMbLE Saccharomyces cerevisiae
Fluoroalcohol-mediated reductive iodonio-Claisen rearrangement: Synthesis of complex
Hem Raj Khatri,Hai Nguyen,James K. Dunaway,Jianglong Zhu
《化学科学与工程前沿（英文）》 2015年 第9卷 第3期 页码 359-368 doi: 10.1007/s11705-015-1530-6
关键词： hypervalent iodine allylation fluoroalcohol Claisen rearrangement heterocycles
Exploring the cancer genome in the era of next-generation sequencing
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《医学前沿（英文）》 2012年 第6卷 第1期 页码 48-55 doi: 10.1007/s11684-012-0182-x
The emergence of next-generation sequencing technologies has led to dramatic advances in cancer genome studies. The increased efficiency and resolution of next-generation sequencing greatly facilitate the detection of genetic, genomic, and epigenomic alterations, such as single nucleotide mutations, small insertions and deletions, chromosomal rearrangements, copy number variations, and DNA methylation. Comprehensive analysis of cancer genomes through approaches of whole genome, exome, and transcriptome sequencing has significantly improved the understanding of cancer biology, diagnosis, and therapy. The present study briefly reviews the recent pioneering studies on cancer genome sequencing and provides an unprecedented insight into the landscape of genomic alterations in human sporadic cancers.
关键词： next-generation sequencing cancer genome whole genome sequencing exome transcriptome
The development and application of genome editing technology in ruminants: a review
Mengke YUAN, Yuanpeng GAO, Jing HAN, Teng WU, Jingcheng ZHANG, Yongke WEI, Yong ZHANG
《农业科学与工程前沿（英文）》 2020年 第7卷 第2期 页码 171-180 doi: 10.15302/J-FASE-2019302
Transgenic ruminants are a valuable resource for both animal breeding and biomedical research. The development of transgenic breeding is proceeding slowly, because it suffers from low efficiency of gene transfer and possible safety problems from uncontrolled random integration. However, new breeding methods combined with genome editing and somatic cell nuclear transfer or microinjection can offer an economic and efficient way to produce gene-edited ruminants, which can serve as bioreactors or have improved disease resistance, animal welfare and product quality. Recent advances in precise genome editing technologies, especially clustered regularly interspaced short palindromic repeat (CRISPR)/Cas9 nucleases, are enabling the systematic development of gene-edited ruminant production. This review covers the development of gene-edited ruminants, the particulars of site-specific engineered nucleases and the state of the art and new insights into practical applications and social acceptance of genome editing technology in ruminants. It is concluded that the production of gene-edited ruminants is feasible and through improvements in genome editing technology it is possible to help feed the world.
关键词： bioreactors breeding engineered endonucleases genome editing ruminants
Lei WANG, Jing LIU
《能源前沿（英文）》 2013年 第7卷 第3期 页码 317-332 doi: 10.1007/s11708-013-0271-9
关键词： liquid metal material genome energy material material discovery advanced material room-temperature liquid alloy thermodynamics phase diagram
《农业科学与工程前沿（英文）》 2022年 第9卷 第2期 页码 309-311 doi: 10.15302/J-FASE-2022440
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《医学前沿（英文）》 2018年 第12卷 第1期 页码 23-33 doi: 10.1007/s11684-017-0607-7
Two decades have passed since the first bacterial whole-genome sequencing, which provides new opportunity for microbial genome. Consequently, considerable genetic diversity encoded by bacterial genomes and among the strains in the same species has been revealed. In recent years, genome sequencing techniques and bioinformatics have developed rapidly, which has resulted in transformation and expedited the application of strategy and methodology for bacterial genome comparison used in dissection of infectious disease epidemics. Bacterial whole-genome sequencing and bioinformatic computing allow genotyping to satisfy the requirements of epidemiological study in disease control. In this review, we outline the significance and summarize the roles of bacterial genome sequencing in the context of bacterial disease control and prevention. We discuss the applications of bacterial genome sequencing in outbreak detection, source tracing, transmission mode discovery, and new epidemic clone identification. Wide applications of genome sequencing and data sharing in infectious disease surveillance networks will considerably promote outbreak detection and early warning to prevent the dissemination of bacterial diseases.
关键词： genome sequencing genomic epidemiology bacteria surveillance infectious diseases
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《医学前沿（英文）》 2015年 第9卷 第2期 页码 146-161 doi: 10.1007/s11684-014-0373-8
Obesity prevalence has increased in recent years. Lifestyle change fuels obesity, but genetic factors cause more than 50% of average variations in obesity. The advent of genome-wide association studies (GWAS) has hastened the progress of polygenic obesity research. As of this writing, more than 73 obesity susceptibility loci have been identified in ethnic groups through GWAS. The identified loci explain only 2% to 4% of obesity heritability, thereby indicating that a large proportion of loci remain undiscovered. Thus, the next step is to identify and confirm novel loci, which may exhibit smaller effects and lower allele frequencies than established loci. However, achieving these tasks has been difficult for researchers. GWAS help researchers discover the causal loci. Moreover, numerous biological studies have been performed on the polygenic effects on obesity, such as studies on fat mass- and obesity-associated gene (FTO), but the role of these polygenic effects in the mechanism of obesity remains unclear. Thus, obesity-causing variations should be identified, and insights into the biology of polygenic effects on obesity are needed.
关键词： obesity genetics genome-wide association studies body mass index fat mass- and obesity-associated gene
A brief review of genome editing technology for generating animal models
Haoyi WANG, Sen WU, Mario R. CAPECCHI, Rudolf JAENISCH
《农业科学与工程前沿（英文）》 2020年 第7卷 第2期 页码 123-128 doi: 10.15302/J-FASE-2019309
The recent development of genome editing technologies has given researchers unprecedented power to alter DNA sequences at chosen genomic loci, thereby generating various genetically edited animal models. This mini-review briefly summarizes the development of major genome editing tools, focusing on the application of these tools to generate animal models in multiple species.
Yaofeng ZHAO, Caixia GAO, Sen WU
《农业科学与工程前沿（英文）》 2020年 第7卷 第2期 页码 121-122 doi: 10.15302/J-FASE-2019317
《医学前沿（英文）》 页码 889-906 doi: 10.1007/s11684-023-0994-x
关键词： primary central nervous system lymphoma whole-genome sequencing TMSB4X copy number variation gene mutation
Embryo-mediated genome editing for accelerated genetic improvement of livestock
Zachariah MCLEAN, Björn OBACK, Götz LAIBLE
《农业科学与工程前沿（英文）》 2020年 第7卷 第2期 页码 148-160 doi: 10.15302/J-FASE-2019305
Selecting beneficial DNA variants is the main goal of animal breeding. However, this process is inherently inefficient because each animal only carries a fraction of all desirable variants. Genome editing technology with its ability to directly introduce beneficial sequence variants offers new opportunities to modernize animal breeding by overcoming this biological limitation and accelerating genetic gains. To realize rapid genetic gain, precise edits need to be introduced into genomically-selected embryos, which minimizes the genetic lag. However, embryo-mediated precision editing by homology-directed repair (HDR) mechanisms is currently an inefficient process that often produces mosaic embryos and greatly limits the numbers of available edited embryos. This review provides a summary of genome editing in bovine embryos and proposes an embryo-mediated accelerated breeding scheme that overcomes the present efficiency limitations of HDR editing in bovine embryos. It integrates embryo-based genomic selection with precise multi-editing and uses embryonic cloning with elite edited blastomeres or embryonic pluripotent stem cells to resolve mosaicism, enable multiplex editing and multiply rare elite genotypes. Such a breeding strategy would enable a more targeted, accelerated approach for livestock improvement that allows stacking of beneficial variants, even including novel traits from outside the breeding population, in the most recent elite genetic background, essentially within a single generation.
关键词： animal breeding cattle cloning CRISPR/Cas9 cytoplasmic injection embryo genome editing germline chimaeras HDR livestock improvement TALENs
Rumin YANG, Fanhui MENG, Xiao WANG, Yaquan WANG
《化学科学与工程前沿（英文）》 2011年 第5卷 第4期 页码 401-408 doi: 10.1007/s11705-011-1129-5
关键词： silicalite-1 cyclohexanone oxime vapor phase Beckmann rearrangement ?-caprolactam
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《医学前沿（英文）》 2013年 第7卷 第3期 页码 280-289 doi: 10.1007/s11684-013-0265-3
Many gene fusions have been recognized as important diagnostic and/or prognostic markers in human malignancies. In recent years, novel gene fusions have been identified in cases without prior knowledge of the genetic background. Accompanied by a powerful computational data analysis method, new genome-wide screening approaches were used to detect cryptic genomic aberrations. This review focused on advanced genome-wide screening approaches in fusion gene identification, such as microarray-based approaches, next-generation sequencing, and NanoString nCounter gene expression system. The fundamental rationale and strategy for fusion gene identification using each biotech platform are also discussed.
关键词： gene fusion cancer microarray next-generation sequencing NanoString nCounter system
标题 作者 时间 类型 操作
Conserved gene arrangement in the mitochondrial genomes of barklouse families Stenopsocidae and Psocidae
Xiaochen LIU, Hu LI, Yao CAI, Fan SONG, John-James WILSON, Wanzhi CAI
期刊论文
Improving prodeoxyviolacein production via multiplex SCRaMbLE iterative cycles
Juan Wang, Bin Jia, Zexiong Xie, Yunxiang Li, Yingjin Yuan
期刊论文
Fluoroalcohol-mediated reductive iodonio-Claisen rearrangement: Synthesis of complex
Hem Raj Khatri,Hai Nguyen,James K. Dunaway,Jianglong Zhu
期刊论文
The development and application of genome editing technology in ruminants: a review
Mengke YUAN, Yuanpeng GAO, Jing HAN, Teng WU, Jingcheng ZHANG, Yongke WEI, Yong ZHANG
期刊论文
Liquid metal material genome: Initiation of a new research track towards discovery of advanced energy
Lei WANG, Jing LIU
期刊论文
GENOME EDITING: A GROUND BREAKING RESEARCH HAS BEEN RANKED TOP 10 ENGINEERING FRONTS FROM 2017 TO 2021
期刊论文
Transforming bacterial disease surveillance and investigation using whole-genome sequence to probe the
null
期刊论文
Advancement in genetic variants conferring obesity susceptibility from genome-wide association studies
null
期刊论文
A brief review of genome editing technology for generating animal models
Haoyi WANG, Sen WU, Mario R. CAPECCHI, Rudolf JAENISCH
期刊论文
Highlights of special issue “Genome Editing in Agriculture: Technology, Applications and Regulations”
Yaofeng ZHAO, Caixia GAO, Sen WU
期刊论文
Analysis of the genomic landscape of primary central nervous system lymphoma using whole-genome sequencing
期刊论文
Embryo-mediated genome editing for accelerated genetic improvement of livestock
Zachariah MCLEAN, Björn OBACK, Götz LAIBLE
期刊论文
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